Simplified HPLC Method for Urinary and Circulating Creatinine
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چکیده
منابع مشابه
Simplified HPLC method for urinary and circulating creatinine.
HPLC with various detection methods, including ultraviolet absorbance, is frequently used to separate and measure creatinine and creatine in serum, plasma, and urine. Current HPLC and other analytical methods for the measurement of creatinine, including capillary electrophoresis and gas chromatography–mass spectrometry, have been reviewed recently (1 ). In 1990, Paroni et al. (2 ) reported a ca...
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Mouse models are frequently used to study renal function. However, mouse serum contains chromagens that interfere with standard picric acid-based assays for serum creatinine. Several alternative methods exist for serum creatinine measurements, including assay by high-performance liquid chromatography (HPLC), but only one has been adapted to mouse serum. Creatinine was measured in serum by aceto...
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The increasing numbers of investigations into metabolic processes have necessitated the adaptation to automated principles of a method whereby the efficacy of quantitative urine collections can be tested. The estimation of creatinine (creatine anhydride) for this purpose has several aJvantages over other methods which have been used. Creatinine excretion is fairly constant from day to day (Foli...
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J1HE INHALATION of lead and its salts, in the form of fumes or dust, has long been a problem in the industrial usage of lead. Awareness of this problem has led to several methods of determining lead exposure in suspected persons (4). One method which has become widely used is the determination of urinary lead levels. A method which is most suitable was described by Cholak et a!. (2, 3), and the...
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The determination of creatinine by HPLC was performed by direct injection of serum onto the column, and after ultrafiltration of the sample, and the results were compared. A modified weak cation exchange column was used for HPLC. This eliminates the protein displacement effect and the Gibbs-Donnan effect, thus permitting a rapid direct analysis. Three of the four ultrafiltration methods gave al...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.024141